Biological Safety Cabinet

Class I Biological Safety Cabinet

• Provides personnel and environmental protection

• It is similar in air movement to a chemical fume but usually has a limited fixed work access opening and the exhaust air must be HEPA filtered, to protect the environment

• Designed for general microbial research with low and moderate-risk agents.

• The work zone, which type is much smaller than a chemical fume hood, is geared to working with biological whose process/ producers dictates the use of moderate amounts of volatile liquids, that are suitable for recirculation cabinets.

Class II Biological Safety Cabinet

• The work is being done within the cabinet

• The facility requirement based on bio safety levels

• The location of the cabinet within the laboratory itself

• Installation requirements of the cabinet

• Exhaust/ supply system(s) to both the

• laboratory and the cabinet, whether or not the cabinet is to be vented

Class III Biological Safety Cabinet

• Designed to work with bio safety level 4

• Microbiological agents

• Provides maximum protection to the

• environment and the worker

• A gas-light enclosure with a no opening

• Completely sealed, viewing window

• Access to the interior through a double-door pass-through interchange box (such as an autoclave) that can be decontaminated between uses

• Reversing the process allows for safe removal of material from the Class III bio safety cabinet